Anti-Actinin-α1 / 2 / 3 / 4 Antibody

This rabbit polyclonal antibody from Antibodies.com is raised against α-actinin family members and detects endogenous levels of total Actinin-α1/2/3/4 protein across multiple applications including Western Blot, Immunohistochemistry and ELISA. The immunogen is a synthetic peptide corresponding to amino acids 30-60 of human Actinin-α1, and the reagent has been affinity-purified from rabbit antiserum by affinity chromatography using the epitope-specific immunogen, reaching approximately 95% purity by SDS-PAGE. It is supplied as an unconjugated, liquid polyclonal IgG formulation in a 50 µL volume, intended for research use only. The antibody shows reactivity with Human, Mouse and Rat samples, making it suitable for cross-species studies of cytoskeletal α-actinin isoforms encoded by ACTN1, ACTN2, ACTN3 and ACTN4, which belong to the spectrin gene superfamily and function as F-actin cross-linking proteins in both non-muscle and muscle cell types. Recommended working conditions follow standard Antibodies.com polyclonal antibody protocols, with storage at −20 °C in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide to preserve activity through multiple freeze/thaw cycles. The product ships from the Antibodies.com logistics network under the standard research-use guarantee and is appropriate for laboratories investigating cytoskeletal organization, Z-disc architecture in striated muscle, cell adhesion junctions and α-actinin–associated signaling pathways.