Anti-Actinin-α1 Antibody

Raised in rabbit as a polyclonal reagent against the cytoskeletal actin-bundling protein α-actinin 1, this unconjugated antibody from Antibodies.com is supplied as a 100 µl vial at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. The antibody recognizes an epitope conserved across human, mouse and rat samples, supporting Western blot detection of the target band as well as immunohistochemical localization of α-actinin 1 in fixed tissue sections where it marks sarcomeric Z-discs, focal adhesions and cell–cell contact zones. Storage at –20 °C in single-use aliquots preserves activity; working aliquots kept refrigerated at 4 °C are stable for routine short-term use, and the glycerol cryoprotectant guards against freeze–thaw damage. Researchers studying striated muscle architecture, cytoskeletal remodeling, mechanotransduction and cell-junction biology use this reagent in both in vitro cell lysate workflows and in vivo tissue analysis, and the unconjugated format leaves the choice of fluorophore or HRP secondary open for immunofluorescence, chromogenic IHC or chemiluminescent Western blot workflows.