Anti-Actin Antibody

Produced in rabbit as a polyclonal reagent, this unconjugated primary antibody from Antibodies.com targets Actin, a highly conserved cytoskeletal protein routinely used as an internal loading control in western blot workflows. Supplied at 100 µl, it is validated for WB and IHC applications on human samples. Actin expression remains relatively constant across most cell and tissue types, making it an effective reference standard for normalizing protein loading across gels and verifying equal transfer in membrane-based assays. Researchers commonly detect the target band at approximately 42 kDa, the expected molecular weight of beta-actin. For optimal stability, store aliquots at -20°C and avoid repeated freeze-thaw cycles to preserve binding activity. The unconjugated format allows flexible pairing with reporter-conjugated secondary antibodies, supporting both chromogenic and fluorescent detection strategies in standard immunodetection protocols. For research use only.