Anti-ACTA2 Antibody

This rabbit polyclonal IgG reagent from Antibodies.com targets ACTA2, the smooth muscle alpha-actin isoform expressed in vascular smooth muscle cells, myofibroblasts, and contractile stromal populations. The unconjugated, affinity-purified antibody is supplied in a 50 µl vial prepared in a phosphate-buffered saline formulation containing glycerol and 0.02% sodium azide as a preservative, so it can be paired with any HRP-, fluorophore-, or biotin-labelled secondary for chromogenic, fluorescence, or chemiluminescent detection. Validated across western blot (WB), immunohistochemistry (IHC), and immunoprecipitation (IP) workflows, the polyclonal response recognises multiple epitopes on the ~42 kDa target, supporting consistent signal on human, mouse, and rat samples including formalin-fixed tissue sections and detergent-lysed cell extracts. Researchers commonly deploy it for studies of vascular remodelling, aortic aneurysm and dissection pathology, cardiac fibrosis, organ scarring, wound healing, tumour-stroma interactions, and smooth muscle lineage tracing. The unconjugated format keeps the reagent compatible with alkaline phosphatase, peroxidase, Alexa Fluor, and streptavidin-based detection systems. Vials are dispatched cold and should be aliquoted on arrival, then stored at -20°C to limit repeated freeze-thaw exposure on working stocks. A short dilution titration is recommended when switching between sample types or fixation conditions, since target abundance and tissue processing can shift the working concentration required for clean background.