Anti-ACSS2 Antibody

ACSS2 (Acyl-CoA Synthetase Short Chain Family Member 2) is an enzyme that catalyzes the ATP-dependent conversion of acetate to acetyl-CoA, a reaction central to lipid biosynthesis, histone acetylation, and cellular energy metabolism. This unconjugated rabbit polyclonal antibody from Antibodies.com recognizes the human ACSS2 protein, a roughly 78 kDa cytoplasmic and nuclear enzyme, and is validated for Western blot (WB) and immunohistochemistry (IHC) workflows. It supports studies of cancer cell metabolism, hypoxia-driven gene regulation, and neuronal acetyl-CoA signaling, where ACSS2 expression is often elevated.

Supplied as 100 µl of affinity-purified reagent, the antibody is raised in rabbit and intended to detect the full-length ACSS2 protein in human cell lysates and in formalin-fixed paraffin-embedded tissue sections. Researchers investigating acetate-fueled tumor growth, lipid droplet formation, or chromatin acetylation in human cancer cell lines can use it alongside metabolic labeling assays to probe ACSS2 levels under different oxygen or nutrient conditions. The polyclonal format provides multi-epitope recognition, which is helpful when probing post-translationally modified or variant forms of the enzyme.

For sample preparation, lysates prepared in standard RIPA buffer with protease inhibitors are compatible with downstream Western blot detection, and citrate-based antigen retrieval (pH 6.0) is recommended for IHC on paraffin sections. Each lot is quality-controlled for reactivity against human ACSS2, supporting reproducible results in basic research and translational studies focused on metabolic disease and oncology. As a research-use-only reagent, this antibody is intended for laboratory investigation and is not approved for diagnostic or therapeutic applications.