Anti-ACIN1 Antibody

The antibody targets ACIN1 (Apoptotic Chromatin Condensation Inducer 1), a nuclear protein that participates in RNA splicing and chromatin remodeling during programmed cell death. This polyclonal reagent from Antibodies.com was raised in rabbit and is supplied as an unconjugated 100 µl volume suitable for Western blot and immunohistochemistry workflows on human and mouse samples.

Real-world applications include studies of caspase-mediated cleavage during apoptosis, investigations of splicing factor complex assembly, and characterization of nuclear morphology changes in response to DNA damage. In Western blot, the antibody detects the full-length ~150 kDa ACIN1 band, while IHC on formalin-fixed paraffin-embedded tissue reveals nuclear staining patterns in proliferative and apoptotic compartments. Researchers studying hematological malignancies, neurodegeneration, and stress-induced apoptosis pathways have used anti-ACIN1 reagents to track the transition from intact ACIN1 to its cleaved apoptotic fragment.

The unconjugated format allows flexible pairing with HRP, fluorescent, or biotinylated secondary detection reagents depending on the assay platform. Cross-reactivity has not been formally characterized beyond the listed human and mouse reactivity, so users working with rat, primate, or other vertebrate samples should perform their own validation. Storage at -20 °C in small aliquots is recommended to limit freeze-thaw cycles of the polyclonal preparation.