Anti-ACIN1 Antibody

A rabbit polyclonal IgG reagent supplied by Antibodies.com detects ACIN1, the 220 kDa nuclear factor that drives caspase-3-activated chromatin condensation and also functions as a component of the spliceosome-associated ASAP complex. The antibody is raised against an internal region of the human ACIN1 protein and cross-reacts with mouse and rat orthologs, supporting comparative apoptosis and RNA-processing studies across standard laboratory models.

Validated for western blot, the reagent is shipped as 200 µl of unconjugated liquid in a single vial, giving laboratories the flexibility to pair it with HRP, biotin, or fluorescent secondaries. Whole-cell or tissue lysates resolved by SDS-PAGE and transferred to nitrocellulose or PVDF membranes yield a characteristic band near 220 kDa, which corresponds to full-length AcinusL. Optimal working dilutions should be determined empirically for each sample preparation.

Aliquot immediately upon arrival and store at -20°C; repeated freeze-thaw cycles reduce activity, and conjugated detection reagents prepared from this stock should be protected from light. The unconjugated IgG format also supports immunoprecipitation workflows that pull down the ASAP ribonucleoprotein assembly. Research use only.