Anti-AChE Antibody

This unconjugated rabbit polyclonal antibody from Antibodies.com targets acetylcholinesterase (AChE), the enzyme that terminates cholinergic neurotransmission by hydrolyzing acetylcholine at synaptic clefts and neuromuscular junctions. The reagent is supplied in a 100 µl volume and is validated for Western Blot and Immunofluorescence workflows on human samples, making it useful for research in neurodegeneration, cholinergic signaling, organophosphate toxicology, and myasthenia gravis.

Raised in rabbit as a polyclonal reagent, this AChE antibody offers batch-to-batch epitope diversity that supports detection across multiple regions of the target protein. Its unconjugated format lets researchers pair the primary with their preferred secondary labeling chemistry, including HRP, fluorophore, or biotin conjugates, depending on the assay platform. Because acetylcholinesterase is expressed in multiple splice variants and exists as both membrane-bound and soluble forms, polyclonal recognition is often preferred when probing tissue lysates, cultured neurons, or transfected cell lines.

Typical applications include characterizing AChE distribution in brain and skeletal muscle tissue sections by Immunofluorescence, confirming protein expression and apparent molecular weight by Western Blot, and supporting investigations into anti-cholinesterase compounds, organophosphate exposure models, and cholinesterase inhibitor pharmacology. Store refrigerated and aliquot upon receipt to limit freeze-thaw cycles if the reagent is intended for long-term use. For research use only.