Anti-ACAT1 Antibody

This primary antibody from Antibodies.com is a rabbit polyclonal reagent raised against ACAT1 (acetyl-CoA acetyltransferase 1), the mitochondrial thiolase enzyme that condenses two acetyl-CoA molecules into acetoacetyl-CoA. The unconjugated IgG preparation is supplied as 100 µL of polyclonal antiserum validated for western blot, immunohistochemistry, and immunofluorescence workflows, with demonstrated reactivity against human, mouse, and rat samples.

ACAT1 localizes to the mitochondrial matrix where it participates in ketone body biosynthesis, fatty acid β-oxidation, and the mevalonate isoprenoid pathway. Researchers studying mitochondrial energetics, hepatic ketogenesis, or inherited beta-ketothiolase deficiency use this reagent to track endogenous protein levels across tissue sections and cell lysates. For western blot, denaturing SDS-PAGE conditions preserve the recognized epitope; for IHC and IF, antigen retrieval methods appropriate to formalin-fixed paraffin-embedded tissue or cultured cells yield cytoplasmic and mitochondrial staining consistent with the target's known subcellular distribution.

Store refrigerated in the supplied formulation to preserve antibody activity through the rated shelf life; for long-term use, aliquot and freeze at -20°C to limit freeze-thaw cycles. Each lot is quality controlled for titer and specificity prior to release by Antibodies.com.