Anti-ACAA2 Antibody

This rabbit polyclonal antibody targets ACAA2 (Acetyl-CoA Acyltransferase 2), a mitochondrial 3-ketoacyl-CoA thiolase that catalyzes the final thiolytic step of mitochondrial fatty acid beta-oxidation. Produced by Antibodies.com, the reagent is raised in rabbit, supplied as an unconjugated 100 µl volume in a single-use vial, and validated for Western blot (WB) and immunohistochemistry (IHC) workflows. The antibody reacts with human and mouse samples, supporting studies of peroxisomal and mitochondrial lipid metabolism, ketogenesis, and inherited metabolic disorders in translational research.

ACAA2 expression is commonly examined in liver, cardiac, and skeletal muscle tissues, where beta-oxidation flux is highest. Researchers investigating fatty acid oxidation defects, mitochondrial dysfunction, or metabolic reprogramming in cancer use this reagent to probe protein levels across cell lines and tissue sections. The polyclonal format provides broader epitope coverage than monoclonal alternatives, which aids detection of multiple ACAA2 isoforms and post-translational modifications in cell culture and tissue-derived samples.

For Western blot, the antibody is applied to whole-cell or mitochondrial lysates separated by SDS-PAGE, with signal developed via HRP- or fluorescence-conjugated secondary antibodies. For IHC, the reagent is used on formalin-fixed, paraffin-embedded sections following standard antigen retrieval. To preserve activity, refrigerate the vial upon arrival, store in small aliquots to limit freeze-thaw cycles, and protect from light until use.