Anti-ABCG2 Antibody

Affinity-purified from rabbit serum, this unconjugated polyclonal IgG reagent from Antibodies.com recognizes human ABCG2, the ATP-binding cassette transporter also known as BCRP (Breast Cancer Resistance Protein). It is supplied as 100 µl of liquid antibody and is validated for western blot detection of endogenous transporter levels in human samples. ABCG2 is widely studied for its role in xenobiotic efflux and multidrug resistance in cancer cell lines, as well as for its protective activity at physiological barriers including the placenta, the intestinal epithelium, and the blood-brain barrier. The polyclonal format is useful for recognizing multiple epitopes on the transporter, an advantage when target expression is low or heterogeneous across different tissue preparations. Researchers working with side population stem cells, which depend on ABCG2-mediated Hoechst dye exclusion, also rely on this reagent to confirm transporter expression by western blot in fractionated cell lysates following culture under stem cell–permissive conditions. Because the antibody is supplied unconjugated, end users retain full flexibility to pair it with horseradish peroxidase- or fluorophore-conjugated secondary antibodies, allowing each laboratory to match the detection chemistry to its preferred substrate, membrane type, and imaging platform.