Anti-ABCB7 Antibody

The Antibodies.com rabbit polyclonal reagent targets ABCB7, a mitochondrial inner-membrane ATP-binding cassette transporter that contributes to cytosolic iron-sulfur cluster biogenesis. Loss-of-function mutations in the encoding gene cause X-linked sideroblastic anemia with cerebellar ataxia, and the protein is therefore a marker of interest in studies of mitochondrial iron homeostasis, heme biosynthesis, and Friedreich ataxia-related pathways.

Supplied as 100 µl of an unconjugated, affinity-purified antiserum formulated at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a cryoprotectant, this primary antibody is validated for Western blot detection of the human, mouse, and rat ABCB7 ortholog. The glycerol-containing buffer is suitable for aliquoting and storage at -20 °C with minimal freeze-thaw damage, and the vial format allows the reagent to be diluted directly into standard TBST-based blocking and wash buffers. Researchers studying mitochondrial transporter function, cytosolic iron-sulfur cluster assembly, and iron-overload disease models commonly use this reagent to probe ABCB7 expression in cell lysates, mitochondrial isolates, and tissue homogenates separated by reducing SDS-PAGE.