Anti-A2M Antibody

This polyclonal reagent from Antibodies.com targets alpha-2-macroglobulin (A2M), a large tetrameric serum glycoprotein that functions as a broad-spectrum protease inhibitor. Raised in rabbit hosts, the unconjugated IgG preparation enables flexible assay design across both western blot and immunohistochemistry workflows on human-derived samples. Researchers studying hepatic function, acute-phase responses, and neurodegeneration frequently rely on A2M detection because circulating levels shift during inflammation and the protein also interacts with amyloid-beta in Alzheimer's research.

Validated applications cover western blot and immunohistochemistry, with the antibody supplied as a 100 µl aliquot sufficient for multiple staining or blotting experiments depending on dilution. On western blot the reagent detects the intact A2M tetramer near 720 kDa under non-reducing conditions, while immunohistochemistry localizes the glycoprotein to hepatocytes and other A2M-expressing tissues. The preparation is intended for research use only and performs best with freshly prepared tissue sections or freshly lysed protein, which helps preserve antigenicity for downstream visualization.

Reactivity is confirmed for human samples, making this reagent appropriate for translational studies on A2M biology and clinical biomarker workup. Store the vial refrigerated between uses and avoid repeated freeze-thaw cycles to maintain binding activity across the working shelf life.