Anti-β-actin Antibody

Generated in mouse as a monoclonal reagent and supplied at 100 µl, this unconjugated primary antibody from Antibodies.com recognizes β-actin, a ~42 kDa cytoskeletal housekeeping protein routinely used as an internal loading control. The reagent is validated for western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) workflows, and has been characterized for reactivity toward zebrafish samples in addition to standard mammalian targets. Each vial ships in a buffered solution ready to be diluted according to the user's chosen detection protocol, supporting consistent normalization across protein gels, fixed tissue sections, and cultured cell preparations. Recommended handling practices include refrigerating the vial between uses, protecting it from light, and limiting repeated freeze-thaw cycles to preserve binding activity over the antibody's working shelf life.