Anti-α-SMA Antibody

This Mouse monoclonal antibody from Antibodies.com targets alpha-smooth muscle actin (α-SMA), a 42 kDa actin isoform expressed in vascular smooth muscle cells, myofibroblasts, and pericytes. Supplied as an unconjugated reagent in a 50 µl vial, the antibody is validated for western blot and immunohistochemistry on Human, Mouse, and Rat samples.

α-SMA staining is widely used to identify activated fibroblasts in fibrosis models, mark cancer-associated fibroblasts in tumor stroma studies, and follow smooth muscle differentiation during wound healing and vascular remodeling experiments. The unconjugated format allows flexible pairing with HRP-, biotin-, or fluorophore-labelled secondary antibodies, supporting both chromogenic and immunofluorescence detection workflows in fixed tissue sections and isolated cell preparations.

For best results, aliquot the antibody upon arrival and store at -20°C in working volumes to limit freeze-thaw cycles. Short-term storage at 4°C (up to one month) is acceptable for active use, and brief centrifugation before opening helps recover the full volume from the vial.