Anti-α-SMA Antibody

α-SMA (alpha smooth muscle actin) is a 42 kDa actin isoform expressed abundantly in vascular smooth muscle and serves as a canonical marker for myofibroblast differentiation across fibrosis, wound healing, and tumor stroma research. This polyclonal reagent from Antibodies.com was raised in rabbit and is supplied as 100 µl of unconjugated antiserum. The antibody recognizes Human, Mouse, and Rat samples, supporting consistent use across orthologous rodent disease models and primary human tissue sections. Validated applications include Western blot for expression profiling of contractile phenotype markers, immunohistochemistry for localization in fibrotic lesions and vascular walls, and ELISA for quantitative studies. For best results, aliquot upon first use to limit freeze-thaw cycles and preserve reagent integrity.