Anti-4E-BP1 (phospho-T46) Antibody

Phosphorylation of 4E-BP1 at threonine 46 is a priming event in the mTORC1 signaling cascade that controls cap-dependent translation initiation. This unconjugated rabbit polyclonal antibody, supplied by Antibodies.com, specifically detects the phospho-T46 epitope and is validated for western blot and immunohistochemistry workflows on human, mouse, and rat samples. The reagent is supplied as 100 µl of antibody at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide, approximately pH 7.2, and can be diluted directly into standard blocking and incubation buffers for immunoblotting or section staining. Because the reagent selectively recognizes the phospho-T46 modification rather than total 4E-BP1 protein, it is useful for studies of nutrient sensing, growth-factor signaling, and translational control in cultured cells and tissue sections. Researchers comparing basal and stimulated conditions can pair this reagent with antibodies against total 4E-BP1 to assess activation status, and with upstream kinase markers such as phospho-Akt or phospho-S6K to map mTOR pathway activity across experimental time courses.