Anti-4E-BP1 (phospho-T37) Antibody

This rabbit polyclonal primary antibody from Antibodies.com selectively detects 4E-BP1 phosphorylated at threonine 37, a regulatory node within the mTORC1 signaling cascade that governs cap-dependent translation initiation. The reagent is supplied as 100 µL at 1 mg/mL in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.2, unconjugated and validated for Western blot and immunohistochemistry workflows. Cross-reactivity has been confirmed in Human, Mouse, and Rat samples, supporting use across cell line, primary tissue, and animal model studies. Threonine 37 acts as a priming site whose phosphorylation precedes modification at T46, T70, and S65, so this reagent is commonly used to monitor early mTOR activation in response to nutrient, growth factor, or stress cues. Researchers employ it to investigate translational control in cancer cell signaling, insulin and metabolic signaling studies, autophagy flux experiments, and neuronal plasticity work where dysregulated cap-dependent translation is implicated. For long-term storage, freeze at -20°C in working aliquots to preserve phospho-specific reactivity; avoid repeated freeze-thaw cycles and protect from light to maintain antibody integrity. The polyclonal format provides recognition of multiple epitopes surrounding the phospho-T37 site, increasing tolerance for minor sample variations compared with monoclonal alternatives.