Anti-4E-BP1 (Ab-64) Antibody

This unconjugated Rabbit polyclonal antibody from Antibodies.com targets 4E-BP1, a translational repressor that binds eukaryotic initiation factor 4E and serves as a primary downstream read-out of mTORC1 kinase activity. The reagent ships as 100 µl of polyclonal antiserum and is validated for western blot and immunohistochemistry across Human, Mouse, and Rat sample backgrounds, supporting studies of cap-dependent translation control, growth factor signaling, autophagy, and tumor cell proliferation.

Phosphorylation of 4E-BP1 at multiple serine and threonine residues releases the bound eIF4E, enabling cap-dependent translation of structured mRNAs including those encoding cyclins, c-Myc, and VEGF. Researchers therefore use anti-4E-BP1 antibodies to monitor both total expression and phosphorylation shifts in response to nutrient status, insulin, and oncogenic cues. The polyclonal format gives broader epitope coverage than monoclonal alternatives, which helps with consistent detection across endogenous protein isoforms in whole-cell lysates and formalin-fixed tissue sections.

For long-term retention, the antibody should be aliquoted and frozen, avoiding repeated freeze-thaw cycles that erode polyclonal activity. The unconjugated format lets laboratories conjugate the reagent to their preferred HRP, fluorophore, or bead system for downstream applications such as immunofluorescence on fixed cells, immunoprecipitation of endogenous 4E-BP1 complexes, and chromogenic IHC staining of formalin-fixed paraffin-embedded tumor and tissue specimens.