Anti-20S Proteasome alpha 6 Antibody

Raised in rabbit, this unconjugated polyclonal antibody binds the human 20S proteasome alpha 6 subunit, designated PSMA6, one of the seven alpha-type subunits that assemble into the outer ring of the 20S proteolytic core particle. The PSMA6 subunit contributes to the regulatory gate that controls entry of polyubiquitinated substrates into the catalytic chamber, and changes in its expression track with altered proteasome activity in many cell types. Antibodies.com supplies the reagent as a 100 µl liquid aliquot at working dilutions appropriate for Western blot (WB) and immunofluorescence (IF) workflows, covering both denatured lysate screening and imaging of fixed cells or tissue sections. Because the antibody is unconjugated, it can be paired with HRP, biotin, fluorophore-conjugated, or alkaline phosphatase secondary reagents chosen by the end user, supporting chemiluminescent, colorimetric, and fluorescence-based detection platforms. Reactivity is reported against human samples, and the polyclonal format raises the probability of recognizing the target across more than one epitope, even where modest variation in the alpha 6 ring is present.