Anti-17beta-HSD4 Antibody

Raised in rabbits, this unconjugated polyclonal antibody targets the peroxisomal enzyme 17β-hydroxysteroid dehydrogenase type 4 (17beta-HSD4), also known as D-bifunctional protein. Distributed by Antibodies.com for research use only, the reagent is validated for western blot, immunohistochemistry, and immunofluorescence workflows on human samples. It supports studies of peroxisomal fatty acid β-oxidation, steroid hormone catabolism, and D-bifunctional protein deficiency, a peroxisomal biogenesis disorder linked to HSD17B4 mutations. Because the polyclonal preparation recognizes multiple epitopes on the target, it can detect truncated splice variants and point mutants that often escape monoclonal antibodies, which aids genotype-phenotype analyses in patient-derived fibroblasts. The product ships as 100 µl of liquid antiserum aliquoted from a single immunisation lot, and pairs with standard HRP- or fluorophore-conjugated secondary reagents for chromogenic or fluorescence detection. Routine applications include band visualization at the expected molecular weight on PVDF membranes, staining of paraffin-embedded and frozen tissue sections, and subcellular localization studies that highlight the peroxisomal compartment.