Anti-15-PGDH Antibody

Targeting 15-hydroxyprostaglandin dehydrogenase (15-PGDH, encoded by the HPGD gene), this unconjugated rabbit polyclonal primary antibody from Antibodies.com is validated for Western blot, immunohistochemistry, and immunofluorescence workflows. The enzyme serves as the key catabolic step that converts prostaglandin E2 (PGE2) into its inactive 15-keto metabolite, making it a frequent focus in studies of inflammation resolution, parturition, and tumor biology where loss of 15-PGDH activity is associated with elevated PGE2 signaling.

Raised in rabbit and provided in a 100 µl vial, the reagent recognizes human 15-PGDH and is unconjugated, allowing users to pair it with their preferred HRP- or fluorophore-conjugated secondary antibody. Recommended storage is at -20 °C in single-use aliquots to limit freeze-thaw cycles. For Western blot applications, the antibody detects endogenous 15-PGDH near its expected molecular weight of approximately 29 kDa; for IHC on formalin-fixed paraffin-embedded tissue, standard antigen retrieval methods followed by chromogenic or fluorescent detection produce cytoplasmic staining consistent with the known peroxisomal and cytoplasmic distribution of the native enzyme.

Because 15-PGDH is widely investigated as a candidate tumor suppressor in colon, lung, and breast carcinoma models and as a downstream effector in cytokine-driven prostaglandin regulation, this reagent is also commonly cited in placental and endometrial tissue research. Researchers should empirically determine the optimal dilution for their specific sample type and include appropriate positive and negative controls expressing endogenous HPGD.