Anti-14-3-3 ζ Antibody

This polyclonal reagent from Antibodies.com targets the 14-3-3 ζ (zeta) regulatory protein, which in humans is encoded by the YWHAZ gene. Members of the 14-3-3 family function as scaffold and adaptor proteins that recognize phosphorylated serine and threonine residues on client partners, influencing intracellular signaling, apoptosis control, mitotic checkpoint progression, and cellular metabolism.

Raised in rabbit, the antibody is provided as an unconjugated liquid fraction of 100 µl in a single-use vial format, validated for western blot, immunohistochemistry, and ELISA workflows. It cross-reacts with human, mouse, and rat samples, supporting comparative studies across standard model organisms and primary tissue specimens. Because the polyclonal backbone presents multiple epitope specificities against the ~28 kDa 14-3-3 ζ target, the reagent is well suited to tracking changes in expression, subcellular localization, and post-translational modification state.

Researchers apply 14-3-3 ζ antibodies to investigations of cancer cell signaling, neuronal stress responses, and kinase-pathway analysis, where altered 14-3-3 ζ abundance has been linked to tumor progression and metabolic disease. For short-term use, store refrigerated at 4 °C; for longer projects, aliquot and freeze at -20 °C, minimizing repeated freeze-thaw cycles to preserve antibody activity.