Anti-14-3-3 θ/YWHAQ Antibody

Phosphorylation-state-specific detection of the 14-3-3 θ scaffold protein, encoded by the YWHAQ gene, is enabled by this rabbit polyclonal reagent supplied by Antibodies.com. The antibody recognizes the serine 232 phosphorylated epitope, a regulatory site whose modification alters client-binding affinity within the broader 14-3-3 signaling network that governs cell cycle progression, apoptosis, and cellular stress responses.

Validated for Western blot (WB), the unconjugated reagent is supplied as a 100 µl liquid aliquot at 1 mg/ml in phosphate buffered saline containing 0.05% sodium azide at approximately pH 7.3. It cross-reacts with samples from human, rat, and mouse origin, supporting comparative studies across standard laboratory models. For handling, refrigerate at 4 °C for short-term use; for longer storage, freeze at -20 °C and aliquot on first thaw to avoid repeated freeze-thaw cycles that can compromise antibody titer.

Because 14-3-3 θ operates as a dimeric adapter that sequesters phosphorylated client proteins in the cytoplasm, monitoring S232 phosphorylation offers a window into pathway activation linked to cell cycle checkpoints, apoptotic signaling, and stress-responsive kinase cascades. Researchers working on neurodegeneration, mitotic regulation, or metabolic signaling pathways will find this phospho-specific antibody a complementary tool alongside total 14-3-3 θ immunoblots, allowing the activated pool of the protein to be resolved from baseline expression in cell lysates.