Anti-β-Actin Antibody

This mouse monoclonal antibody recognizes the conserved 42 kDa cytoskeletal protein β-actin, a standard loading-control target in immunoblotting and a routine cytoplasmic reference in cell-based imaging. Supplied by Antibodies.com as an unconjugated reagent, the antibody is provided at 50 µl volume and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows. Clonality is monoclonal with mouse as the host species, supporting reproducible lot-to-lot specificity for cytoskeletal labeling across human, rat, mouse, monkey, and canine samples. Store at -20 °C in single-use aliquots and protect from light; avoid repeated freeze-thaw cycles to preserve binding activity. Researchers typically deploy this reagent to confirm equal protein loading on Western blot membranes, to reveal cytoplasmic architecture in fixed tissue sections via immunohistochemistry, and to counterstain cellular structures during immunofluorescence imaging of cultured cells. The unconjugated format enables flexible pairing with user-selected HRP-conjugated secondary antibodies for chemiluminescent detection or with fluorophore-labeled secondary reagents for multicolor fluorescence microscopy, accommodating diverse experimental pipelines in cell biology, neuroscience, and cancer research laboratories.