Anti-β-Actin (4D3) Antibody

HRP-conjugated mouse monoclonal antibody targeting β-Actin, the 42 kDa cytoskeletal protein routinely used as a loading control in protein expression studies. Distributed by Antibodies.com, the 4D3 clone is supplied at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide at approximately pH 7.2, in a 100 µl vial. The reagent is validated for Western blot and immunohistochemistry workflows, and the horseradish peroxidase conjugate enables direct chromogenic detection in tissue sections without the need for a secondary antibody step. Verified reactivity spans human, mouse, and rat samples, with broader recognition reported across mammalian tissues, making this antibody a useful housekeeping-protein reference for normalisation across blots from mixed-species studies. Aliquot upon arrival to limit freeze-thaw cycles, store refrigerated between uses, and protect from light to preserve conjugate activity over the working shelf life.