Anti-β-Actin (4D3) Antibody

Offered through Antibodies.com, this Mouse monoclonal reagent targets β-Actin and is supplied as a 50 µl aliquot at 1 mg/ml in phosphate buffered saline (PBS) with 15 mM sodium azide at approximately pH 7.2. The clone4D3 was raised against recombinant full-length human β-Actin and detects the endogenous protein at the expected molecular weight of ~42 kDa. It is validated for Western blot (WB) and immunohistochemistry (IHC) applications, making it a versatile loading control and cytoskeletal marker for routine work. The antibody is unconjugated and was affinity-purified from mouse antiserum using affinity chromatography with the epitope-specific immunogen, achieving a purity of ~95% by SDS-PAGE. Host species is Mouse with monoclonal clonality, and reactivity has been confirmed against Human, Mouse, and Rat samples, with broader coverage reported across mammalian species. The product form, buffer composition, and affinity purification together support reproducible performance in both WB and IHC workflows. Researchers should aliquot upon arrival and avoid repeated freeze-thaw cycles to preserve activity, as is standard for antibodies stored in PBS with sodium azide; the conjugate is unconjugated, allowing flexible pairing with secondary antibodies of choice. This reagent is intended for research use only and is not for diagnostic or therapeutic applications.