Anti-α-SMA Antibody

This recombinant rabbit monoclonal antibody from Antibodies.com targets α-smooth muscle actin (α-SMA), a 42 kDa actin isoform expressed in smooth muscle cells, myofibroblasts, pericytes, and cells undergoing epithelial-to-mesenchymal transition. Supplied as an unconjugated reagent in 50 µl aliquots, it is formulated in 1× TBS pH 7.4 with 1% BSA and 40% glycerol as stabilizers and 0.05% sodium azide as preservative, supporting reproducible handling across standard immunohistochemistry, Western blot, and flow cytometry workflows.

Validated applications include Western blot, immunohistochemistry, and flow cytometry, with confirmed reactivity against human, mouse, rat, and zebrafish α-SMA. This broad species coverage makes the reagent useful for comparative studies across mammalian models and zebrafish developmental biology, including investigations of vascular smooth muscle differentiation, cardiac and skeletal muscle tissue organization, organ fibrosis, wound repair, and tumor stroma biology where activated fibroblasts are quantified in tissue sections and dissociated cell preparations.

Because the antibody is unconjugated, it can be paired with a secondary reagent of the researcher's choice for fluorescent or chromogenic detection, allowing integration into multicolor flow cytometry panels and standard IHC platforms alike. The recombinant monoclonal production format supports lot-to-lot consistency, which is particularly valuable for serial tissue studies and quantitative densitometry on Western blots where antibody reproducibility directly affects signal interpretation.