AllTaq PCR Core Kit (5000 u)

The AllTaq PCR Core Kit from QIAGEN is designed to simplify end-point PCR by providing a single protocol that works across all templates without optimization. The versatile system supports duplex reactions and amplifies difficult, GC-rich, or long targets up to 9 kb, all with the same workflow. A built-in visual pipetting control uses an optional orange Master Mix Tracer and blue Template Tracer that turn green when combined, confirming correct reaction setup. These inert dyes also serve as gel loading and tracking dyes to streamline downstream analysis.

Stringent hot-start functionality is delivered through a unique guard molecule and antibody that keep DNA polymerase inactive until heat activation (2 min, 95°C), preventing non-specific amplification and primer-dimer formation. The kit enables true room-temperature setup with 4°C storability, allowing automated workflows, and achieves results with a 45-minute total run time. It is highly sensitive, capable of reliably detecting as low as a single target molecule.

Contents include AllTaq Polymerase, AllTaq PCR Buffer, dNTP Mix, Template Tracer, Master Mix Tracer, Q-Solution, MgCl2, and RNase-Free Water.