Allprotect Tissue Reagent Kit (100ml)

This tissue reagent from QIAGEN is designed for the immediate stabilization of DNA, RNA and proteins in freshly harvested tissue samples. By submerging tissue in the reagent at the point of collection, the in vivo molecular profile is preserved and degradation is prevented, supporting reliable downstream analysis without the need for hazardous liquid nitrogen or dry ice. The reagent works at ambient temperatures: stabilized tissues can be stored at room temperature (15–25°C) for up to 7 days, or at 2–8°C for up to 6 months without showing degradation of DNA, RNA or protein. For longer-term archiving, tissues can be kept at –20°C or –80°C.

A key advantage is that all three analytes can be purified from the same sample, eliminating the variation inherent in preparing DNA, RNA and protein from different sources. The reagent is intended for use with QIAGEN's AllPrep DNA/RNA/Protein Mini Kit for simultaneous purification, and is also compatible with DNeasy, QIAamp, RNeasy and Qproteome kits for individual analyte preparation. Functional protein suitable for enzyme assays can be obtained using the Qproteome Mammalian Protein Prep Kit. The reagent is intended for human and animal tissues, and can also be used with plant tissues.

For waxy plant leaves, scratching the surface helps the reagent permeate. Recommended homogenization tools are the TissueRuptor or TissueLyser; rotor–stator homogenizers with a metal probe are not recommended if proteins will subsequently be purified. Because the reagent causes tissues to shrink, it is not suitable for morphological studies. Excess reagent can be removed by dabbing the sample on a paper towel, and residual reagent does not inhibit purification or downstream applications. Multiple freeze–thaw cycles do not affect RNA or protein analysis, though protein activity assays should be validated individually.