Allprep PowerViral DNA/RNA Kit (50)

This viral nucleic acid isolation kit from QIAGEN is designed for fast and easy purification of viral and bacterial total nucleic acids, even from samples high in PCR inhibitors. It is intended for extracting high-quality material from stool and complex samples in microbiome research, and supports sample types including processed waste water, stool, biosolids and gut material.

The AllPrep PowerViral DNA/RNA Kit uses silica spin filter columns with a binding capacity of up to 40 µg per spin filter and supports a throughput of 1–24 samples. Processing is achieved through chemical lysis and/or bead beating, using optional 0.1 mm glass beads. For stool samples or samples with a solid matrix, lysis is carried out using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer; for virus extraction from water samples and samples that do not require dispersion, the bead-beating step may be skipped in favour of chemical lysis only. Each run or prep takes 45 minutes, and the kit should be stored at room temperature (15–30°C).

An integrated Inhibitor Removal Technology ensures complete removal of inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells that are abundant in stool. Nucleic acids are eluted in RNase-free water and are ready to use in downstream applications including PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing and next-generation sequencing. The kit was previously sold by MO BIO as the PowerViral Environmental DNA/RNA Kit.