pQE-TriSystem Vector DNA

The pQE-TriSystem Vector from QIAGEN is a plasmid vector designed for the expression and purification of recombinant proteins. It features a triple fusion tag system that combines a His6-tag, a SUMO tag, and a FLAG tag.

The His6-tag enables purification by metal affinity chromatography, while the SUMO tag is described as enhancing solubility and helping to reduce aggregation of expressed proteins. The FLAG tag provides an additional purification and detection option.

According to the QIAexpressionist handbook, the vector is suited for expression in bacterial, mammalian and insect cells. It is designed to work with a range of E. coli strains and supports both small- and large-scale protein production. With multiple cloning sites and efficient expression controls, the vector is presented as suitable for protein production and structural studies in molecular biology.