Mouse XpressRef Universal Total RNA

This universal total RNA reference from QIAGEN is designed for use as a positive control in gene expression detection methods. The Mouse XpressRef Universal Total RNA sample is intended to contain the broadest possible representation of genes from a specific organism, and equivalent reference preparations are available for human, mouse, and rat.

The mouse RNA is prepared from several male and female mice (Balb/c strain), using whole bodies without fur, and is combined with RNA from various organs to ensure the broadest gene coverage. It is prepared on an industrial scale using a modified guanidine isothiocyanate technique, which ensures high-quality RNA.

XpressRef Universal Total RNA samples are useful for troubleshooting real-time or semi-quantitative RT-PCR gene expression analyses, particularly when the gene of interest is difficult to detect, and for optimizing real-time RT-PCR gene expression assays by enabling the determination of amplification efficiencies when experimental RNA samples are precious. The reference RNA may also serve as an internal normalization control for two-color microarray systems or as a troubleshooting tool for single-color microarray systems, making it highly suited for the standardization and troubleshooting of semi-quantitative and real-time RT-PCR workflows.