Mitomycin C, 10 mg

From STEMCELL Technologies, this Mitomycin C antibiotic functions as a double-stranded DNA alkylating agent, supplied as a crystalline solid with a purity of ≥ 98%. The molecule (C15H18N4O5; molecular weight 334.3 g/mol) covalently crosslinks DNA, inhibiting DNA synthesis and cell proliferation through reductive activation involving low pH or enzymes such as NAD(P)H:quinone oxidoreductase (DT-diaphorase) or NADH cytochrome c reductase.

In stem cell workflows, the product is used to mitotically inactivate mouse embryonic fibroblasts (MEFs) for use as feeder cell layers in embryonic stem cell co-culture systems, supporting maintenance and self-renewal. In cancer research applications, it selectively inhibits DNA synthesis and mutagenesis, stimulates genetic recombination, chromosome breakage, and sister chromatid exchange, and induces DNA repair.

Product documentation provides solubility data for DMSO, absolute ethanol, and PBS (pH 7.2), along with stock solution guidance. A California Proposition 65 warning notes that Mitomycin C is known to the State of California to cause cancer. The product is for research use only and not intended for human or animal diagnostic or therapeutic uses.