Hygromycin, 100 mg

A standard selection reagent for cell culture and genetic engineering, this aminoglycoside antibiotic from STEMCELL Technologies inhibits protein synthesis by interfering with ribosomal translocation, enabling the isolation of stably transfected lines carrying the hygromycin B resistance gene (hph).

The 100 mg vial format supports routine selection in bacteria, yeast, and mammalian systems, with working concentrations typically ranging from 50 to 200 µg/mL depending on cell type and construct. Selection is generally completed within 7 to 14 days.

Reconstituted stocks can be stored frozen at -20 °C in aliquots to limit freeze-thaw cycles and preserve activity over multi-week experiments. Its broad-spectrum activity makes it a practical alternative or complement to puromycin, G418, and blasticidin in dual-selection protocols.