Ligation Solution

A ready-to-use enzymatic reagent from QIAGEN, the Ligation Solution is formulated for efficient adapter attachment during NGS library construction workflows. Supplied as a single-use vial containing the ligation buffer and enzyme mix optimized for joining NGS adapters to fragmented double-stranded DNA, this reagent streamlines the adapter ligation step in both manual and automated library prep protocols.

Used within QIAGEN's Universal NGS consumable lineup, the solution supports robust ligation across varied input DNA qualities, including fragmented genomic DNA, amplicons, and cell-free DNA templates. Each vial delivers consistent performance at standard reaction conditions, with the ligation reaction typically carried out at room temperature for 15 to 30 minutes before cleanup with a bead-based or column-based purification method.

The reagent integrates into targeted sequencing, whole-genome sequencing, and custom capture workflows, including those built around QIAseq chemistry. Researchers can combine the Ligation Solution with QIAGEN adapters, indexing kits, and amplification reagents to build multiplexed libraries for Illumina sequencing platforms. Storage at -20 degrees C preserves enzymatic activity through multiple freeze-thaw cycles without substantial loss of ligation efficiency, making it a reliable choice for high-throughput genomics laboratories running routine NGS pipelines.