Hu PSC Naive qPCR Type 1, 96 wells

Confirm and monitor the naive pluripotent state in human pluripotent stem cells with this 96-well gene expression profiling kit from STEMCELL Technologies. The panel interrogates a curated set of naive-state markers — including KLF4, KLF5, TFCP2L1, TBX3, ESRRB, DNMT3L, DPPA3, and DPPA5 — alongside housekeeping reference genes for ΔΔCt normalization. This Type 1 configuration distinguishes cells maintained under classical naive ground-state conditions from primed or reset intermediate populations.

Each plate is pre-configured with human-specific primer assays dried into individual wells, removing the need to dispense individual primer sets. Workflow: isolate total RNA from cultured hPSCs, reverse-transcribe to cDNA, add PCR master mix and template into wells, and run on any standard real-time cycler such as QuantStudio, CFX96, or LightCycler. Reaction volume per well is 20 µL.

Common research applications include verifying successful primed-to-naive resetting, lot-to-lot and passage-to-passage consistency checks, and side-by-side comparison of naive maintenance media formulations. The kit supports qPCR-based characterization workflows in cell culture and stem cell research laboratories. Store the plate at -20 °C, protect from light, and limit freeze-thaw cycles to preserve assay performance. Research use only; not intended for diagnostic procedures.