Hu PSC Endoderm qPCR Type 3, 96 wells

For definitive endoderm characterization in human pluripotent stem cell workflows, the STEMCELL Technologies Hu PSC Endoderm qPCR Type 3 array provides gene expression screening in a pre-aliquoted primer plate containing 96 reactions. The kit targets endoderm-lineage transcription factors and developmental markers such as SOX17, FOXA2, HNF4A, GATA4, CXCR4, and CER1, alongside housekeeping reference genes for normalization. Each well is pre-loaded with human-specific primer pairs validated for SYBR-based qPCR detection, allowing direct addition of cDNA template to a standard 20 uL reaction. Researchers typically prepare cDNA from total RNA isolated across staged PSC-to-endoderm protocols (often 100-500 ng per reverse transcription reaction) and add 1-2 uL of template per well. The plate supports rapid profiling of differentiation efficiency using human-specific primer sequences, and the array is intended for research use only, stored at -20 degrees C with protection from light to preserve dried primer stability. The format is compatible with standard real-time PCR instruments including Applied Biosystems, Bio-Rad, and Roche platforms.