FERA Solution

Combining enzymatic DNA fragmentation, end-repair, and 3'-A-tailing in a single reaction, QIAGEN's FERA Solution streamlines the front end of next-generation sequencing library preparation. The reagent mixture is optimized for QIAseq targeted DNA workflows and accepts a broad input range, typically 1 ng to 1 µg of DNA per reaction, supporting applications from low-input cell-free DNA preps to high-yield genomic DNA isolations. By replacing three sequential enzymatic steps with one 30-minute incubation, the solution reduces hands-on bench time, limits sample loss through fewer purification steps, and delivers a uniform fragment-size distribution that supports reliable downstream adapter ligation and target enrichment.

Researchers use this reagent for targeted resequencing, variant discovery, somatic mutation profiling, and rare-variant detection on Illumina sequencing platforms. The product is supplied in a multi-reaction vial format and shipped on cold packs; it should be stored at -20 °C upon arrival, with working aliquots recommended to limit freeze-thaw cycles and preserve enzymatic activity over the kit's shelf life.

FERA Solution integrates seamlessly into QIAGEN's broader QIAseq library construction pipeline, where it pairs with QIAseq indexed adapters and targeted panel probes to produce sequencing-ready libraries. Marked for research use only, the reagent supports reproducible library yields across operators, instruments, and run dates, helping translational genomics teams standardize their front-end workflow.