Manufactured by Antibodies.com as a rabbit polyclonal reagent, this unconjugated primary antibody targets triiodothyronine, the biologically active thyroid hormone responsible for regulating metabolic rate, cardiac output, body temperature, and cellular growth. The antibody is purified through Protein A chromatography and supplied at 1 mg per vial, supporting both assay development and validation runs.

Triiodothyronine (T3) levels serve as a key biomarker in clinical endocrinology, where elevated or suppressed concentrations point to hyperthyroidism, hypothyroidism, Graves' disease, Hashimoto's thyroiditis, and various thyroid neoplasms. In laboratory research, T3 quantification supports studies of the hypothalamic-pituitary-thyroid axis, deiodinase enzyme activity, thyroid hormone receptor signaling, and metabolic disease models such as obesity and insulin resistance. Veterinary diagnostics also rely on T3 measurement, particularly in canine and feline thyroid panels.

ELISA is the principal application for this reagent; the polyclonal antibody recognizes multiple epitopes on the T3 molecule, which improves tolerance to minor sample matrix variations. Beyond immunoassay workflows, the antibody is used in vitro cell-based hormone response experiments, lateral flow immunoassays, chemiluminescence platforms, and as a capture or detection reagent in custom assay development.

Because the antibody is unconjugated, researchers can conjugate it to a preferred reporter enzyme, fluorophore, or biotin label. For short-term use, refrigerate upon arrival; for long-term stability, freeze at low temperature and aliquot to limit repeated freeze-thaw cycles. Protect the vial from prolonged light exposure to preserve binding activity.

This primary antibody fits laboratory workflows across academic endocrinology, pharmaceutical screening for thyroid-disrupting compounds, clinical chemistry reference method development, and veterinary diagnostic kit manufacturing. The Protein A purified preparation provides low background and high specificity, supporting reproducible performance across batches.

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