XC Buffer, 10x (192ul)

The 10x concentrated XC Buffer from QIAGEN is supplied as a 192 ul vial of concentrated stock, intended for next-generation sequencing library preparation workflows. Diluted 1:10 with nuclease-free water immediately before use, the buffer supports enzymatic steps such as end repair, adapter ligation, and amplification while maintaining consistent ionic conditions for downstream hybridization or capture-based target enrichment protocols.

Each vial yields roughly 1.9 mL of 1x working buffer, enough for approximately ten standard library construction reactions depending on the protocol and reaction volume requirements. The 10x stock format reduces shipping and storage footprint while keeping the working solution fresh at the bench.

Storage recommendations include refrigeration between uses, protection from light, and equilibration to working temperature prior to pipetting. The reagent integrates with QIAGEN Universal NGS consumables and bead-based cleanup chemistries for production of sequencing-ready libraries. Researchers should minimize freeze-thaw cycles and handle with nuclease-free technique to preserve performance across library prep batches.