Treponema pallidum (TpN15)-HRP Antigen

This liquid-phase antigen conjugate from Antibodies.com pairs recombinant Treponema pallidum TpN15 with horseradish peroxidase (HRP), giving researchers an enzyme-labeled detection reagent ready for direct use in syphilis serology workflows. TpN15 is a 15 kDa membrane lipoprotein of T. pallidum subsp. pallidum and is one of the established recombinant targets used in treponemal immunoassays to confirm exposure to the causative agent of syphilis.

Because the antigen is already conjugated to HRP, it can be applied as a detection reagent in ELISA and Western blot without the need for a labeled secondary antibody, simplifying assay setup. The conjugate is supplied as a 1 mL liquid preparation in a vial suitable for immediate dilution into working buffer, and is intended for research use in diagnostic development, seroprevalence studies, and immunology research.

Typical use cases include screening recombinant antigen panels for syphilis antibody detection, validating treponemal Western blot strips, and comparing TpN15 reactivity against other T. pallidum immunogens such as TpN17, TpN47, and Tpp47. Researchers handling the conjugate should store it refrigerated and protect it from light to preserve HRP activity, and avoid repeated freeze-thaw cycles of working aliquots.