Thyroxine (T4)-HRP Antigen

HRP-Labeled Thyroxine Antigen for Immunoassay Workflows

A directly conjugated antigen reagent for laboratories developing or running competitive immunoassays against thyroxine (T4), the principal tetraiodinated hormone produced by the thyroid gland. The T4 molecule is covalently linked to horseradish peroxidase (HRP), so the conjugate emits a chromogenic, luminogenic, or fluorogenic signal—depending on the substrate chosen—without requiring a separate secondary antibody incubation step.

Supplied as a liquid in a 1 ml vial, the conjugate fits directly into plate-based protocols for ELISA and similar solid-phase binding assays. It serves as the labeled competitor in competitive formats, where sample T4 and the HRP-labeled T4 compete for binding sites on a capture antibody, producing an inverse relationship between signal intensity and analyte concentration.

Common laboratory applications include endocrine research, thyroid function screening assays, and method development for veterinary or clinical T4 measurement. The conjugate should be stored protected from light and refrigerated to preserve HRP enzymatic activity; repeated freeze-thaw cycles will degrade the label and reduce signal output.

Manufactured by Antibodies.com, this conjugate is produced under standardized conjugation chemistry to support consistent labeling density and reproducible assay performance across production lots.