SeaBlock/EIA/WB w/ Tris

This liquid blocking buffer from Antibodies.com is formulated with Tris for saturation of unbound binding sites on microplate wells and Western Blot membranes prior to antibody probing. The non-mammalian protein base reduces cross-reactivity that arises when mammalian-reactive secondary antibodies are paired with conventional milk or BSA blockers, a frequent issue in immunochemistry workflows that probe human or rodent samples. Tris buffering helps maintain stable pH across incubation and washing steps, and the ready-to-use 500 mL presentation means no reconstitution or dilution step is required before pipetting. The reagent is applied directly as a plate or membrane blocker in ELISA and Western Blot protocols, and it doubles as a diluent for primary and secondary antibodies within those same workflows. Because the blocker is protein-based rather than detergent-based, it preserves native antibody binding activity while suppressing nonspecific background on nitrocellulose, PVDF, and polystyrene surfaces.