SeaBlock EIA/WB w/ PBS

A fish-protein-based blocking buffer formulated for sensitive immunoassay workflows. Supplied as a 500ml liquid in PBS, this reagent from Antibodies.com replaces conventional milk or BSA blockers in ELISA and Western Blot protocols, eliminating the cross-reactivity issues that milk-derived casein can introduce with biotin-streptavidin detection systems. The PBS formulation avoids phosphoprotein contamination, preserving recognition by phospho-specific antibodies during immunoblotting, and reduces background on nitrocellulose and PVDF membranes while maintaining target epitope accessibility. Compatible with chemiluminescent, colorimetric, and fluorescent detection chemistries. Refrigerate upon receipt and protect from prolonged light exposure. Each bottle is lot-tested for consistent blocking performance across membrane-based and microplate-based assay platforms.