Recombinant Human DNA Polymerase kappa Protein (Functional)

The Recombinant Human DNA Polymerase kappa (Pol κ) Protein from Antibodies.com is an active, full-length translesion synthesis polymerase expressed for functional research applications. As a Y-family DNA polymerase encoded by the POLK gene, Pol κ plays a critical role in error-prone replication past bulky adducts such as BPDE-DNA lesions and 8-oxo-guanine, supporting studies in DNA damage response, mutagenesis, and genome stability. Researchers commonly deploy this enzyme in functional assays that measure nucleotide incorporation across damaged templates, in WB (Western Blot) analysis for protein detection, and ELISA-based quantification workflows. The protein is supplied as a 50 µg liquid preparation, enabling direct addition to in vitro polymerase reactions. For optimal activity, store at -80°C, avoid repeated freeze-thaw cycles, and aliquot upon first use to preserve enzymatic function. This reagent is intended for research use only and supports investigations into translesion synthesis mechanisms relevant to cancer biology, xeroderma pigmentosum variant (XP-V) studies, and chemotherapeutic resistance pathways.