Rat Immunoglobulin G

Purified from normal rat serum using standard chromatographic methods, this Immunoglobulin G is supplied by Antibodies.com as an unconjugated, lyophilised powder in a 10 mg format. The unconjugated presentation allows end users to perform custom labelling, biotinylation, or fluorophore conjugation depending on downstream requirements.

Common laboratory applications include use as a blocking agent in immunohistochemistry and western blot membranes, where it occupies non-specific binding sites before applying a primary antibody. The preparation is also widely used as a negative isotype control when validating rat-derived monoclonal antibodies, helping confirm that observed signals reflect specific antigen binding rather than non-specific interactions. In ELISA and immunoassay development, purified rat IgG serves as a reference standard for standard curve preparation or as a coating reagent when characterising anti-rat IgG secondary antibodies.

Reconstitute the lyophilised powder in an appropriate buffer; freeze reconstituted aliquots to preserve functional activity across multiple experiments. The lyophilised form stabilises the protein during shipping at ambient conditions, eliminating cold-chain requirements during transit.

Provided for research use only, this preparation is appropriate for cell biology, immunology, and protein chemistry laboratories that require a reliable source of purified rat immunoglobulin for blocking, control, and reference applications.