PBS 0.5MAG Vessel With Ports, 1 Unit

A single-use 0.5MAG vessel with ports from STEMCELL Technologies forms part of the PBS-MINI compact bioreactor system, designed for high-throughput, 3D suspension culture of human pluripotent stem cells (hPSCs) and other cell types. The vessel belongs to the PBS Bioreactor product line.

Operating at a 500 mL culture volume, the vessel delivered consistently high daily fold expansion when hPSC lines (H9 human ES and WLS-1C iPS) were seeded at 3–8 x 10^4 viable cells/mL and cultured for 4 days in mTeSR™3D. End-of-passage viabilities remained high, and after 9 passages, harvested cells showed high OCT-4 and TRA-1-60 expression. hPSC aggregates in the vessel display typical suspension culture morphology: mostly spherical shape, clear edges, even color, and a dimpled or pock-marked appearance, with aggregates typically 350–400 µm in size. Among vessels tested from 2019–2022, the PBS-MINI 0.5 was recommended for robust suspension culture scale-up, maintaining a mean daily fold expansion above 1.2.

The vessel integrates with workflow components including serum-free media for hESC and hiPSC suspension culture and a four-way split cable that connects up to four PBS-MINI MagDrive Bioreactor Base Units to one power source.