Oxycodone-HRP Antigen

Pre-conjugated to horseradish peroxidase for direct enzymatic readout, this oxycodone hapten reagent from Antibodies.com supports competitive immunoassay development targeting opioid analytes. Supplied as a 1 ml liquid in enzyme stabilizing diluent, the conjugate couples the small-molecule target to HRP, eliminating the need for a secondary antibody step in ELISA plate workflows.

In a competitive format, the HRP-labeled hapten competes with free oxycodone in a sample for binding to immobilized anti-oxycodone antibodies, producing an inverse signal relative to analyte concentration. This approach is widely used in forensic toxicology research, workplace drug screening method development, and validation of monoclonal or polyclonal antibody pairs raised against opioid haptens. Because the HRP label is covalently attached to the small-molecule target, the reagent also serves as a tracer in lateral flow assay prototyping and homogeneous solution-phase studies.

The preparation is ready to dilute into assay buffer and titrate against paired capture antibodies. Researchers typically optimize working concentrations by checkerboard titration on microplate wells pre-coated with anti-oxycodone immunoglobulin. The liquid presentation in enzyme stabilizing diluent supports reproducible aliquoting and minimizes freeze-thaw degradation across multi-batch studies.