Human Interleukin 3 (Active)

The Human Interleukin 3 (Active) recombinant cytokine from Antibodies.com is supplied as 10 µg of lyophilized, unconjugated protein in a single vial for research use. Interleukin-3 (IL-3) is a 15 kDa colony-stimulating factor that drives proliferation and differentiation of multipotent hematopoietic progenitors, spanning myeloid, erythroid, megakaryocytic, and mast cell lineages, by engaging the IL-3 receptor alpha chain (CD123) paired with the common beta chain (CD131).

Bioactivity is commonly confirmed in cell-based proliferation assays using factor-dependent lines such as TF-1 or M-07e, with potency expressed as an ED50 derived against an international reference standard. The lyophilized format supports storage at -20°C in a dry environment; after reconstitution in sterile water or assay buffer, researchers typically aliquot and freeze the working solution at -80°C to limit repeated freeze-thaw cycles and preserve functional activity across the shelf life.

Typical research applications include supporting colony-forming unit (CFU) assays in methylcellulose cultures, expanding primary hematopoietic stem and progenitor cells, exploring JAK2/STAT5 signal transduction, and modeling cytokine-driven differentiation in vitro.